Cryptosporidium Pig Genotype II in Immunocompetent Man

6. David MD, Gill MJ. Potential for under-dosing and emergence of resistance in Acinetobacter baumannii during treatment with colistin. rates of resistance to colistin and polymyxin B in subgroups of Acinetobacter baumannii isolates from Korea. Activity of colis-tin against heteroresistant Acinetobacter baumannii and emergence of resistance in an in vitro pharmacokinetic/pharma-codynamic model. Antimicrob Agents Chemother. To the Editor: Protozoan parasites from the genus Cryptosporidium have been described as a cause of diar-rheal disease in immunodeficient and immunocompetent humans worldwide. Although C. hominis and C. parvum (cattle genotype) cause most infections, humans can be infected by several other Cryptosporidium species or genotypes: C. meleagridis; C. deer genotype, skunk genotype, horse genotype, and chipmunk genotype I (1–4). Wild and domestic animals are sources of infection for humans (and other animals) and important contributors to contamination of food and drinking water; many nonhuman Cryptosporidium species or genotypes are detected in untreated water (5). We examined the diversity of Cryptospo-ridium spp. in immunocompetent persons in South Bohemia in the Czech Republic. Diarrheal fecal samples (n = 457) from 203 anonymous immunocom-petent patients <69 years of age with suspected cryptosporidiosis (at least 2 samples/patient/3-day period) were obtained from local health departments and public hospitals in South Bohemia during 2005–2007. Samples were examined for Cryptosporidium oocysts by using aniline-carbol-methyl violet staining and light microscopy at × 1,000 magnification (6). The microscopically positive samples were confirmed by DNA sequencing of the small subunit (SSU) rRNA gene. Total DNA was extracted from 200–300 mg stool by using the QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germa-ny), following the manufacturer's instructions , after previous homogeniza-tion and disruption of oocysts with the fragment of the SSU rRNA gene was amplified by nested PCR according to Jiang et al. (7). Purified PCR products were sequenced in both directions on an ABI3130 sequencer analyzer (Applied Biosystems, Foster City, CA, USA) by using the secondary PCR primers and the BigDye Terminator v3.1 Cycle Se-quencing Kit (Applied Biosystems). Sequences were assembled by using Chromas Pro (www.technelysium. com.au/chromas.html) and aligned with reference sequences using Clust-rRNA gene partial sequences of the 7 patient isolates have been submitted to GenBank (Table). Of the 203 patients, 7 (3.4%) (6 children and 1 adult) had positive results for Cryptosporidium spp. Moreover , all samples obtained from these persons during the 3-day period were Cryptosporidium spp. positive; partial sequences of the Cryptosporidium SSU rRNA gene were obtained from all positive …